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HIV Protocols pp 11–15Cite as

Quantitative HIV Culture

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 17))

Abstract

This procedure is used for establishing cocultures for virus isolation by an endpoint dilution assay in a 96-well tissue culture plate. This is a cell culture assay based on the detection of HIV in patient peripheral blood mononuclear cells (PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.

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References

  1. Dimitrov, D. H., Melnick, J. L., and Hollinger, F. B. (1990) Microculture assay for the isolation of human immunodeficiency virus type 1 and for titration of infected peripheral blood mononuclear cells. J. Clin. Microbiol. 28, 734–737.

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  2. ACTG Virology Manual for HIV Laboratories. (1993) (Hollinger, F.B., ed.) Version 2.0, MIC-1 toMIC-4.

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Author information

Authors and Affiliations

  1. SRA Technologies, Inc., Rockville, MD

    James R. Lane

Authors
  1. James R. Lane
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Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Rockville, MD

    Nelson L. Michael MD, PhD

  2. Henry M. Jackson Foundation, Rockville, MD

    Jerome H. Kim MD

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© 1999 Humana Press Inc., Totowa, NJ

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Cite this protocol

Lane, J.R. (1999). Quantitative HIV Culture. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:11

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  • DOI: https://doi.org/10.1385/0-89603-369-4:11

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-369-6

  • Online ISBN: 978-1-59259-601-0

  • eBook Packages: Springer Protocols

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