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HIV Protocols pp 205-211 | Cite as

Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism

  • Maureen P. Martin
  • Mary Carrington
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 17)

Abstract

The CCR5 gene encodes a cell-surface chemokine receptor molecule, which serves as a coreceptor for macrophage-tropic strains of HIV-1 (1, 2, 3). Mutations in this gene may alter expression or function of the protein product, thereby altering chemokine binding or HIV-1 infection of cells on which the receptor is normally expressed. Indeed, it was recently shown that individuals homozygous for a mutant allele (CCR5-△32) characterized by a 32-bp deletion in the coding region of the CCR5 gene, are relatively resistant to HIV-1 infection (4, 5, 6). This allele causes a frame shift at amino acid 185, and homozygous individuals fail to express detectable cell-surface CCR5 molecules. It is possible that other as-yet unidentified variants play a role in HIV-1 infectivity and outcome. Several additional mutations, most of which are single-base substitutions, have been identified in the coding region of the CCR5 gene using the single-strand conformation polymorphism (SSCP) technique (7).

Keywords

Polymerase Chain Reaction Product Ammonium Persulfate SSCP Analysis Bromphenol Blue CCR5 Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Maureen P. Martin
    • 1
  • Mary Carrington
    • 1
  1. 1.NCI-Frederick Cancer Research and Development CenterFrederick

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