Abstract
A bacterial defense mechanism has proven to be one of the most valuable tools in the modern molecular biology laboratory. Restriction endonucleases and recombinant DNA technology have been invaluable in genome characterization, mapping, sequencing, and amplification. The recognition and excision of unique nucleotide sites has enabled complex DNA manipulation, beginning with molecular cloning and ending in finished, well elucidated nucleotide sequences.
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References
Singer, M. and Berg, P. (1991) Genes & GenomesāA Changing Perspective. University Science Books, Mill Valley, CA, pp. 243ā254.
Roberts, R. J. and Macelis, D. (1994) REBASEāRestriction Enzymes and Methylases. Nucleic Acids Res. 22, 3628,3629.
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Ā© 1997 Humana Press Inc.
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Plasterer, T.N. (1997). MAPDRAW. In: Swindell, S.R. (eds) Sequence Data Analysis Guidebook. Methods In Molecular Medicineā¢, vol 70. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-358-9:241
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DOI: https://doi.org/10.1385/0-89603-358-9:241
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-358-0
Online ISBN: 978-1-59259-556-3
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