Primer Design
  • Phil Taylor
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 70)


Version 1.5 of GeneJockeyII includes two routines for primer design. One of these generates primer pairs for the polymerase chain reaction (PCR), whereas the other generates primers for site-directed mutagenesis (SDM). Individual primers generated for PCR are also highly suitable for sequencing purposes (see Note 1).


Polymerase Chain Reaction Mutant Sequence GnRH Receptor Experienced Polymerase Chain Reaction Consecutive Base 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Lowe, T., Sharetkin, J., Yang, S. Q., and Dieffenbach, K. (1990) A computer-program for selection of oligonucleotide primers for polymerase chain reactions. Nucleic Acids Res. 18, 1757–1761.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Phil Taylor
    • 1
  1. 1.MRC Reproductive Biology UnitCenter for Reproductive BiologyEdinburghUK

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