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Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM)

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Glycoanalysis Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 76))

Abstract

In comparison to the sequencing of other biological macromolecules such as nucleic acids or proteins, the sequencing of oligosaccharides (glycans) of glycoproteins generally requires specialized expertise and facilities Whereas techniques such as nuclear magnetic resonance can give full sequence information (1–3), they are only available in relatively few laboratories and are by no means routine (discussed further in Chapter 11).

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Authors and Affiliations

  1. Oxford Glycosciences, The Quadrant, Abingdon Science Park, Abingdon, UK

    Sally Prime & Tony Merry

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  1. Sally Prime
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  2. Tony Merry
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Editor information

Editors and Affiliations

  1. University College London, UK

    Elizabeth F. Hounsell

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© 1998 Humana Press Inc., Totowa, NJ

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Prime, S., Merry, T. (1998). Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM). In: Hounsell, E.F. (eds) Glycoanalysis Protocols. Methods in Molecular Biology™, vol 76. Humana Press. https://doi.org/10.1385/0-89603-355-4:53

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  • DOI: https://doi.org/10.1385/0-89603-355-4:53

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-355-9

  • Online ISBN: 978-1-59259-562-4

  • eBook Packages: Springer Protocols

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