Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides

  • Barry J. Campbell
  • Jonathan M. Rhodes
Part of the Methods in Molecular Biology™ book series (MIMB, volume 76)

Abstract

The role of mucus in the protection of intestinal, bronchial, nasopharyngeal, and cervical mucosae has long been recognized, but poorly understood. Research has been stimulated by the expectation that an underlying mucus abnormality might be present, not only in diseases such as cystic fibrosis where there is an obvious physical alteration in mucus, but also in conditions such as inflammatory bowel disease, peptic ulceration, and intestinal cancer where mucus abnormalities at present seem more subtle (1). Progress has been relatively slow, however, not least because of the difficulties in obtaining a pure but undamaged mucus glycoprotein (mucin) preparation.

Keywords

Vortex HPLC H2SO4 Galactose Alkalinity 

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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Barry J. Campbell
    • 1
  • Jonathan M. Rhodes
    • 1
  1. 1.Glycobiology Group, Gastroenterology Research Unit, Department of MedicineThe University LiverpoolUK

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