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High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues

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Protein Sequencing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 64))

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Abstract

High-performance liquid chromatography (HPLC) is one of the most common separation techniques in today’s protein chemistry. With the development of diode-array UV detectors for HPLC instruments on-line derivative spectroscopy has become possible and provides an extremely powerful tool for the analysis of peptides and proteins (2). The method of on-line spectral analysis has been particularly useful in the analysis of peptides containing aromatic residues (2). The formation of second derivatives of the absorption maxima leads to further increases in the resolution between spectral differences and allows one to distinguish between different aromatic residues in a peptide. Based on these findings we have utilized HPLC on-line derivative spectroscopy for the analysis of an important posttranslational modification of peptides and proteins (1).

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References

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Author information

Authors and Affiliations

  1. Department of Medicine, Howard Hughes Medical Institute, University of California San Francisco, CA

    Christoph W. Turck

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  1. Christoph W. Turck
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Editor information

Editors and Affiliations

  1. Celltech Therapeutics, Slough, UK

    Bryan John Smith

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© 1997 Humana Press Inc.

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Turck, C.W. (1997). High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 64. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-353-8:109

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  • DOI: https://doi.org/10.1385/0-89603-353-8:109

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-353-5

  • Online ISBN: 978-1-59259-550-1

  • eBook Packages: Springer Protocols

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