Selective Isolation of the Carboxy Terminal Peptide from a Protein
In contrast to N-termmal sequencing, several methods have been used for determination of the C-terminus. They include a thiocyanate degradation procedure (1), methods using carboxypeptidase (2), hydrazinolysis (3), and tritium labeling (4), depending on the nature of the samples.
KeywordsHPLC Agarose Acetonitrile Carboxyl Lysine
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