N-Terminal Protein Ladder Sequencing
In protein ladder sequencing, the amino acid sequence of a peptide is determined by measuring the molecular mass differences between members of a family of fragments produced from the peptide. Adjacent members of this family of sequence-defining peptide fragments differ from one another by one amino acid residue. The molecular masses of the family of peptide fragments are measured by mass spectrometry in a single operation to read-out of the amino acid sequence. The identity of the amino acid residues is assigned by the mass differences between adjacent peaks and the amino acid sequence is determined by the order of their occurrence in the data set.
KeywordsAmino Acid Residue Coupling Reagent Reaction Vial Basic Amino Acid Residue Ladder Sequencing
- 2.Wang, R., Chait, B. T., and Kent, S. B. H. (1994) Protein ladder sequencing towards automation, in Techniques in Protein Chemistry, vol. 5 (Crabb, J. W., ed.), Academic, San Diego, CA, pp. 19–26.Google Scholar
- 4.Hillenkamp, F., Karas, M., Beavis, R. C., and Chait, B. T. (1991) Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers. Anal. Chem. 63, 1193A–1203A.Google Scholar
- 5.Wang, R. and Chart, B. T. (1996) Post-translational modifications analyzed by automated protein ladder sequencing, in Protein and Peptide Analysis by Mass Spectrometry (Chapman, J. R., ed.), Humana, Totowa, NJ (in press).Google Scholar