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N-Terminal Protein Ladder Sequencing

  • Rong Wang
  • Brian T. Chait
Part of the Methods in Molecular Biology™ book series (MIMB, volume 64)

Abstract

In protein ladder sequencing, the amino acid sequence of a peptide is determined by measuring the molecular mass differences between members of a family of fragments produced from the peptide. Adjacent members of this family of sequence-defining peptide fragments differ from one another by one amino acid residue. The molecular masses of the family of peptide fragments are measured by mass spectrometry in a single operation to read-out of the amino acid sequence. The identity of the amino acid residues is assigned by the mass differences between adjacent peaks and the amino acid sequence is determined by the order of their occurrence in the data set.

Keywords

Amino Acid Residue Coupling Reagent Reaction Vial Basic Amino Acid Residue Ladder Sequencing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Rong Wang
    • 1
  • Brian T. Chait
    • 1
  1. 1.Rockefeller UniversityNew York

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