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HSV Amplicons in Gene Therapy

  • Protocol
Herpes Simplex Virus Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 10))

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Abstract

Herpes simplex virus (HSV) amplicons are defective virus vectors capable of introducing amplified foreign genes into variable types of eukaryotic cells, such as fibroblasts, macrophages, glia, and neurons in different organisms including rodents, monkeys, and human (refs. 1–3; reviewed in ref. 4). The defective viruses follow their nondefective counterparts in the ability to infect mitotic, as well as postmitotic cells. This makes them potentially useful vectors for use in nondividing cells, such as in nerve cells. Available retrovirus vectors employed to date for gene therapy require cell division and therefore cannot be used to target neurons.

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Authors and Affiliations

  1. Department of Cell Research and Immunology, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel

    Niza Frenkel & Ronit Sarid

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  1. Niza Frenkel
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  2. Ronit Sarid
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Editors and Affiliations

  1. University of Glasgow, Scotland, UK

    S. Moira Brown  & Alasdair R. MacLean  & 

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© 1998 Humana Press Inc., Totowa, NJ

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Frenkel, N., Sarid, R. (1998). HSV Amplicons in Gene Therapy. In: Brown, S.M., MacLean, A.R. (eds) Herpes Simplex Virus Protocols. Methods in Molecular Medicine, vol 10. Humana Press. https://doi.org/10.1385/0-89603-347-3:227

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  • DOI: https://doi.org/10.1385/0-89603-347-3:227

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-347-4

  • Online ISBN: 978-1-59259-594-5

  • eBook Packages: Springer Protocols

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