HSV Growth, Preparation, and Assay

  • June Harland
  • S. Moira Brown
Part of the Methods in Molecular Medicine book series (MIMM, volume 10)


Whether herpes simplex virus (HSV) is viewed as a pathogen or as a model eukaryotic system, it is virtually certain that any experimental work will require the virus to be grown and assayed. The following chapter is therefore seen as the fundamental first step before embarking on more intellectually and technically challenging technology. Its importance should not however be underestimated. It never fails to surprize us that people who describe themselves as virologists have little understanding of the basic requirements needed to attain a contamination-free, high-titer, low particle:plaque-forming units (PFU) ratio, genetically pure virus stock


Herpes Simplex Virus Virus Stock Newborn Calf Serum Brain Heart Infusion Agar Roller Bottle 
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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • June Harland
    • 1
  • S. Moira Brown
    • 2
  1. 1.Institute of Neurological Sciences, Southern General Hospital, Neurovirology Research LaboratoriesUniversity of GlasgowScotland, UK
  2. 2.Neurovirology Research LaboratoriesUniversity of Glasgow, Institute of Neurological Sciences, Southern General HospitalGlasgowScotland, UK

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