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Protein and Peptide Analysis by Mass Spectrometry pp 57–65Cite as

Mass Spectrometric Quantification of Neuropeptides

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 61))

Abstract

The molecular specificity that is attached to the quantification of an endogenous neuropeptide is an important experimental parameter (1), because the other analytical methods that are commonly used, such as radioimmunoassay (RIA), provide a very high level of detection sensitivity, but only limited molecular specificity (2). The purpose of this chapter is to describe the use of tandem mass spectrometry (MS/MS) methods to increase the specificity of the qualitative and quantitative analysis of endogenous neuropeptides in human tissue extracts.

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References

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Author information

Authors and Affiliations

  1. The Charles B. Stout Neuroscience Mass Spectrometry Laboratory, Departments of Neurology and Biochemistry, University of Tennessee, Memphis, TN

    Dominic M. Desiderio

Authors
  1. Dominic M. Desiderio
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Editor information

Editors and Affiliations

  1. Manchester, UK

    John R. Chapman

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© 1996 Humana Press Inc., Totowa, NJ

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Desiderio, D.M. (1996). Mass Spectrometric Quantification of Neuropeptides. In: Chapman, J.R. (eds) Protein and Peptide Analysis by Mass Spectrometry. Methods in Molecular Biology™, vol 61. Humana Press. https://doi.org/10.1385/0-89603-345-7:57

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  • DOI: https://doi.org/10.1385/0-89603-345-7:57

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-345-0

  • Online ISBN: 978-1-59259-547-1

  • eBook Packages: Springer Protocols

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