Abstract
Described here is one of the most powerful methods of mass spectrometry (MS) for molecular structure analysis, introduced under the acronym “PSD-MALDI” (postsource decay with matrix-assisted laser desorption/ionization) MS (1–6). It has found rapid and wide acceptance (7–11), mainly because of its attractive features, such as high sensitivity, high tolerance of various sample conditions, speed of analysis, and limited instrumental requirements. All major mass spectrometer companies have now extended their product line to support the PSD-MALDI mode for bioorganic structure analysis.
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References
Spengler, B., Kirsch, D., and Kaufmann, R. (1992) Fundamental aspects of postsource decay in matrix-assisted laser desorption mass spectrometry. J. Phys. Chem. 96, 9678–9684.
Kaufmann, R., Spengler, B., and Lutzenkirchen, F. (1993) Mass spectrometric sequencing of linear peptides by product-ion analysis in a reflectron time-of-flight mass spectrometer using matrix-assisted laser desorption ionization. Rapid Commun. Mass Spectrom. 7, 902–910.
Kaufmann, R., Kirsch, D., and Spengler, B. (1994) Sequencing of peptides in a time-of-flight mass spectrometer evaluation of postsource decay following matrix-assisted laser desorption ionization (MALDI) Int. J. Mass Spectrom. Ion Proc. 131, 355–385.
Spengler, B., Kirsch, D., and Kaufmann, R. (1991) Metastable decay of peptides and proteins in matrix assisted laser desorption mass spectrometry. Rapid Commun. Mass Spectrom. 5, 198–202.
Spengler, B. and Kaufmann, R. (1992) Gentle probe for tough molecules: matrix-assisted laser desorption mass spectrometry. Analusis 20, 91–101.
Spengler, B., Kirsch, D., Kaufmann, R., and Jaeger, E. (1992) Peptide sequencing by matrix assisted laser desorption mass spectrometry. Rapid Commun. Mass Spectrom. 6, 105–108.
Yu, W., Vath, J. E., Huberty, M. C., and Martin, S. A. (1993) Identification of the facile gas-phase cleavage of the Asp-Pro and Asp-Xxx peptide bonds in matrix assisted laser desorption time-of-flight mass spectrometry. Anal. Chem. 65, 3015–3023.
Huberty, M. C., Vath, J. E., Yu, W., and Martin, S. A. (1993) Site-specific carbohydrate identification in recombinant proteins using MALD-TOF MS. Anal. Chem. 65, 2791–2800.
Critchley, W. G., Hoyes, J. B., Malsey, A. E., Wolter, M. A., Engels, J. W., and Muth, J. (1994) Structural Analysis of Mixed Base Oligonucleotides Using Negative Ion Matrix Assisted Laser Desorption Ionisation Time of Flight (MALDI-TOF) Mass Spectrometry Metastable Analysis. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 29-June 3, p 140.
Hoyes, J. B., Curbishley, S. G., Doorbar, P., Tatterton, P., and Bateman, R. H. (1994) A New High Performance MALDI-TOF Instrument for PSD and CID Metastable Ion Analysis. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 29-June 3, p 682.
Talbo, G. and Mann, M. (1994) Localization of Phosphorylation Sites in Peptides by MALDI Metastable Decay at the Subpicomole Level. 13th International Mass Spectrometry Conference, Budapest, Hungary, August 29-September 2.
Beavis, R. C. and Bridson, J. N. (1993) Epitaxial protein inclusion in sinapic acid crystals. J. Phys. D. Appl. Phys. 26, 442–447.
Strupat, K., Karas, M., and Hillenkamp, F. (1991) 2,5-Dihydroxybenzoic acid a new matrix for laser desorption-ionization mass spectrometry. Int. J. Mass Spectrom. Ion Proc. 111, 89–102.
Ehring, H., Karas, M., and Hillenkamp, F. (1992) Role of photoionization and photochemistry in ionization processes of organic molecules and relevance for matrix-assisted laser desorption ionization mass spectrometry. Org. Mass Spectrom. 27, 472–480.
Bokelmann, V., Spengler, B., and Kaufmann, R. (1995) Dynamical parameters of ion ejection and ion formation in matrix-assisted laser desorption/ionization. Eur. Mass Spectrom. 1, 81–93.
Spengler, B., Lutzenkirchen, F., Kirsch, D., Deppe, A., and Kaufmann, R. (1994) Structure Analysis of Biopolymers by PSD-MALDI in a Reflectron Time-of-Flight Mass Spectrometer. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 29-June 3, p. 885.
Spengler, B., Lutzenkirchen, F., and Kaufmann, R. (1993) On-target deuteration for peptide sequencing by laser mass spectrometry. Org. Mass Spectrom. 28, 1482–1490.
Spengler, B., Kirsch, D., Kaufmann, R., and Lemoine, J. (1995) Structure analysis of branched oligosaccharides using post-source decay in matrix-assisted laser desorption ionization mass spectrometry. J. Mass Spectrom. 30, 782–787.
Kirsch, D., Rood, H.-A., Kaufmann, R., and Spengler, B. (1994) Ion Stability of Deoxyoligonucleotides and its Implication for PSD-MALDI-MS. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 29-June 3, p 821.
Spengler, B., Pan, Y., Cotter, R. J., and Kan, L.-S. (1990) Molecular weight determination of underivatized oligodeoxyribonucleotides by positive-ion matrix-assisted ultraviolet laser-desorption mass spectrometry. Rapid Commun. Mass Spectrom. 4, 99–102.
Vorm, O., Roepstorff, P., and Mann, M. (1994) Improved resolution and very high sensitivity in MALDI TOF of matrix surfaces made by fast evaporation. Anal. Chem. 66, 3281–3287.
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© 1996 Humana Press Inc., Totowa, NJ
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Spengler, B. (1996). New Instrumental Approaches to Collision-Induced Dissociation Using a Time-of-Flight Instrument. In: Chapman, J.R. (eds) Protein and Peptide Analysis by Mass Spectrometry. Methods in Molecular Biology™, vol 61. Humana Press. https://doi.org/10.1385/0-89603-345-7:43
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DOI: https://doi.org/10.1385/0-89603-345-7:43
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