Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products

  • Anu Suomalainen
  • Ann-Christine Syvänen
Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)


The time and labor required for generating nucleotide sequence information has been significantly reduced since the development of the polymerase chain reaction (PCR). PCR allows the production of sufficient amounts of DNA template in vitro, and consequently, the time-consuming cloning procedure to obtain large enough quantities of DNA for sequencingisavoided. The PCR has transformed nucleotide sequencing into a method that can be considered even in routine diagnostics for sequencing large numbers of individual samples.


Polymerase Chain Reaction Product Affinity Capture Label Mixture Typical Polymerase Chain Reaction Polymerase Chain Reaction Prep 
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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Anu Suomalainen
    • 1
  • Ann-Christine Syvänen
    • 2
  1. 1.Department of Human Molecular GeneticsNational Public Health InstituteHelsinkiFinland
  2. 2.Department of Biochemistry and BiotechnologyRoyal Institute of TechnologyStockholmSweden

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