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Direct DNA Sequencing of PCR Products Using Magnetic Beads

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PCR Sequencing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 65))

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Abstract

The use of magnetic particles in many fields of biochemistry, molecular biology, and medicine has been well documented, and several magnetic particles are now available for diagnostic and cell-separation purposes. The solid-phase approaches have improved robustness by their increased reproducibility with accompanying higher yields. Furthermore, automation has been facilitated since reaction buffers and additional reagents can be rapidly changed without centrifugation or precipitation steps. The introduction of magnetic particles has proven to be an attractive alternative to traditional means of performing DNA sequencing.

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© 1996 Humana Press Inc., Totowa, NJ

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Lundeberg, J., Pettersson, B., Uhlén, M. (1996). Direct DNA Sequencing of PCR Products Using Magnetic Beads. In: Rapley, R. (eds) PCR Sequencing Protocols. Methods in Molecular Biology™, vol 65. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-344-9:57

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  • DOI: https://doi.org/10.1385/0-89603-344-9:57

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-344-3

  • Online ISBN: 978-1-59259-551-8

  • eBook Packages: Springer Protocols

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