Direct DNA Sequencing of PCR Products Using Magnetic Beads
The use of magnetic particles in many fields of biochemistry, molecular biology, and medicine has been well documented, and several magnetic particles are now available for diagnostic and cell-separation purposes. The solid-phase approaches have improved robustness by their increased reproducibility with accompanying higher yields. Furthermore, automation has been facilitated since reaction buffers and additional reagents can be rapidly changed without centrifugation or precipitation steps. The introduction of magnetic particles has proven to be an attractive alternative to traditional means of performing DNA sequencing.
KeywordsParamagnetic Bead Annealing Buffer Fluorescent Label Primer Dilute Stock Solution Free Biotin
- 9.Hedrum, A., Pontén, F., Ren, Z., Lundeberg, J., Pontén, J., and Uhlén, M. (1994) Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples. BioTechniques 17, 1–9.Google Scholar
- 13.Holmberg, A., Fry, G., and Uhlén, M. (1993) Automated preparation of DNA templates for sequencing on the ABI Catalyst robotic workstation, in Automated DNA Sequencing and Analysis Techniques (Venter, C., ed.), Academic, London, pp. 139–145.Google Scholar
- 16.Pettersson, B., Johansson, K.-E., and Uhlén, M. (1994) Sequence analysis of 16S rRNA from Mycoplasmas by direct solid-phase DNA sequencing. Appl. Env. Microbiol. 60(7), 2456–2461.Google Scholar