Abstract
The polymerase chain reaction (PCR) facilitates the rapid in vitro amplification of target DNA segments. Numerous applications have been developed to exploit the vast potential of PCR, and many of these require sequence analysis of the DNA product. This chapter describes the dideoxy chain-termination method for sequencing PCR products cloned into M13 and plasmid vectors.
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References
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Messing, J. (1993) M13 Cloning Vehicles: Their contribution to DNA sequencing, in DNA Sequencing Protocols (Griffin, H. G. and Griffin, A. M., eds.), Humana, Totowa, NJ, pp. 9–22.
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Gerischer, U. and Dürre, P. (1993) Sequencing using custom designed oligonucleotides, in DNA Sequencing Protocols (Griffin, H. G. and Griffin, A. M., eds.), Humana, Totowa, NJ, pp. 75–82.
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© 1996 Humana Press Inc., Totowa, NJ
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Brewis, N. (1996). Sequencing PCR Products Cloned into M13 Vectors. In: Rapley, R. (eds) PCR Sequencing Protocols. Methods in Molecular Biology™, vol 65. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-344-9:185
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DOI: https://doi.org/10.1385/0-89603-344-9:185
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-344-3
Online ISBN: 978-1-59259-551-8
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