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PCR Sequencing Protocols pp 177–184Cite as

Cloning PCR Products for Sequencing in M13 Vectors

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 65))

Abstract

Although numerous methods are now available for direct sequencing of PCR products, cloning of amplified DNA for sequencing in M13 vectors remains an attractive approach because of the high quality of sequence information generated from single-stranded bacteriophage DNA templates.

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References

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Author information

Authors and Affiliations

  1. Department of Molecular Genetics, School of Biological Sciences, University of Auckland, New Zealand

    David Walsh

Authors
  1. David Walsh
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Editor information

Editors and Affiliations

  1. School of Natural Sciences, Coventry University, Coventry, UK

    Ralph Rapley

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© 1996 Humana Press Inc., Totowa, NJ

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Walsh, D. (1996). Cloning PCR Products for Sequencing in M13 Vectors. In: Rapley, R. (eds) PCR Sequencing Protocols. Methods in Molecular Biology™, vol 65. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-344-9:177

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  • DOI: https://doi.org/10.1385/0-89603-344-9:177

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-344-3

  • Online ISBN: 978-1-59259-551-8

  • eBook Packages: Springer Protocols

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