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Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA

  • Mary I. Coolbaugh Murphy
  • Holly A. Hammond
  • C. Thomas Caskey
Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)

Abstract

The use of the polymerase chain reaction (PCR) allows isolation and examination of genomic DNA sequences using specific oligonucleotide primers to amplify a target region with Taq DNA polymerase (1,2). The PCR products can be screened for mutations using molecular techniques, such as allele-specific oligonucleotides (ASO), single-stranded conformation polymorphisms (SSCP), denaturing gel electrophoresis (DDGE), restriction-fragment-length polymorphisms (RPLP), short tandem repeats (STRs), and heteroduplex formation (3,4).

Keywords

Polymerase Chain Reaction Product Polymerase Chain Reaction Primer Disodium EDTA Polymerase Chain Reaction Tube Buffer Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Mary I. Coolbaugh Murphy
    • 1
    • 2
  • Holly A. Hammond
    • 3
  • C. Thomas Caskey
    • 3
  1. 1.University of Texas Health Science CenterHouston
  2. 2.Texas Medical CenterHouston
  3. 3.Merck Research LabsMerck and Co. Inc.West Point

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