Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA

  • Mary I. Coolbaugh Murphy
  • Holly A. Hammond
  • C. Thomas Caskey
Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)


The use of the polymerase chain reaction (PCR) allows isolation and examination of genomic DNA sequences using specific oligonucleotide primers to amplify a target region with Taq DNA polymerase (1,2). The PCR products can be screened for mutations using molecular techniques, such as allele-specific oligonucleotides (ASO), single-stranded conformation polymorphisms (SSCP), denaturing gel electrophoresis (DDGE), restriction-fragment-length polymorphisms (RPLP), short tandem repeats (STRs), and heteroduplex formation (3,4).


Polymerase Chain Reaction Product Polymerase Chain Reaction Primer Disodium EDTA Polymerase Chain Reaction Tube Buffer Chamber 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Mary I. Coolbaugh Murphy
    • 1
    • 2
  • Holly A. Hammond
    • 3
  • C. Thomas Caskey
    • 3
  1. 1.University of Texas Health Science CenterHouston
  2. 2.Texas Medical CenterHouston
  3. 3.Merck Research LabsMerck and Co. Inc.West Point

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