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Preparation and Direct Automated Cycle Sequencing of PCR Products

  • Susan E. Daniels
Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)

Absract

The polymerase chain reaction (PCR) is well known for being a rapid and versatile method for the amplification of defined target DNA sequences. This technique can be applied to a variety of research areas, such as the identification and typing of single nucleotide substitutions of DNA sequence polymorphisms, and genetic mapping (1, 2, 3, 4).

Keywords

Polymerase Chain Reaction Product Cycle Sequencing Microfuge Tube Single Nucleotide Substitution Chain Terminator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Susan E. Daniels
    • 1
  1. 1.Wellcome Trust Centre for Human GeneticsOxfordUK

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