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PCR Sequencing with the Aid of Detergents

  • Protocol
PCR Sequencing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 65))

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Abstract

The polymerase chain reaction (PCR) allows the rapid detection of infectious viruses or other microorganisms as well as the cause of genetic defects. By performing sequence analysis afterward, important additional information on the PCR products is obtained. Often direct sequencing of the PCR products is preferred over the more time-consuming and expensive procedure of cloning prior to sequencing.

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References

  1. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74, 5463–5467.

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  5. Wu, A. M. and Cetta, A. (1975) On the stimulation of viral DNA polymerase activity by nonionic detergent. Biochemistry. 144), 789–795.

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Author information

Authors and Affiliations

  1. Deutsches Primatenzentrum, Abteilung fur Virologie and Immunolgie, Gottingen, Germany

    Harald Petry, Barbara Bachmann, Wolfgang Lüke & Gerhard Hunsmann

Authors
  1. Harald Petry
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  2. Barbara Bachmann
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  3. Wolfgang Lüke
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  4. Gerhard Hunsmann
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Editor information

Editors and Affiliations

  1. School of Natural Sciences, Coventry University, Coventry, UK

    Ralph Rapley

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Copyright information

© 1996 Humana Press Inc., Totowa, NJ

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Petry, H., Bachmann, B., Lüke, W., Hunsmann, G. (1996). PCR Sequencing with the Aid of Detergents. In: Rapley, R. (eds) PCR Sequencing Protocols. Methods in Molecular Biology™, vol 65. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-344-9:105

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  • DOI: https://doi.org/10.1385/0-89603-344-9:105

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-344-3

  • Online ISBN: 978-1-59259-551-8

  • eBook Packages: Springer Protocols

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