Abstract
DNA sequencing involves a specific application of electrophoresis to resolve the linear single-stranded fragments produced during sequencing reactions, which differ in length by a single base pair. This necessitates using an acrylamide gel, usually at a concentration of 4–20%, of at least 40 cm in length and normally 0.4 mm thick.
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Theophilus, B.D.M. (1996). Preparation and Analysis of DNA Sequencing Gels. In: Rapley, R. (eds) PCR Sequencing Protocols. Methods in Molecular Biology™, vol 65. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-344-9:1
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DOI: https://doi.org/10.1385/0-89603-344-9:1
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-344-3
Online ISBN: 978-1-59259-551-8
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