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Preparation and Analysis of DNA Sequencing Gels

  • Bimal D. M. Theophilus
Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)

Abstract

DNA sequencing involves a specific application of electrophoresis to resolve the linear single-stranded fragments produced during sequencing reactions, which differ in length by a single base pair. This necessitates using an acrylamide gel, usually at a concentration of 4–20%, of at least 40 cm in length and normally 0.4 mm thick.

Keywords

Bottom Edge Straight Edge Sticky Tape Adjacent Lane Plate Assembly 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Mizusawa, S., Nishimura, S., and Seela, F. (1986) Improvement of the di-deoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 14, 1319.PubMedCrossRefGoogle Scholar
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    Reed, A. P., Kost, T. A., and Miller, T. J. (1986) Simple improvements in 35S dideoxy sequencing. BioTechniques 4, 306.Google Scholar
  4. 4.
    Wahls, W. P. and Kingzette, M. (1988) No runs, no drips, no errors: a new technique for sealing polyacrylamide gel electrophoresis apparatus. BioTechniques 6, 308.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Bimal D. M. Theophilus
    • 1
  1. 1.Department of HaematologyThe Birmingham Children’s HospitalBirminghamUK

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