Preparation and Analysis of DNA Sequencing Gels

  • Bimal D. M. Theophilus
Part of the Methods in Molecular Biology™ book series (MIMB, volume 65)


DNA sequencing involves a specific application of electrophoresis to resolve the linear single-stranded fragments produced during sequencing reactions, which differ in length by a single base pair. This necessitates using an acrylamide gel, usually at a concentration of 4–20%, of at least 40 cm in length and normally 0.4 mm thick.


Bottom Edge Straight Edge Sticky Tape Adjacent Lane Plate Assembly 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Bimal D. M. Theophilus
    • 1
  1. 1.Department of HaematologyThe Birmingham Children’s HospitalBirminghamUK

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