Preparation and Analysis of DNA Sequencing Gels
DNA sequencing involves a specific application of electrophoresis to resolve the linear single-stranded fragments produced during sequencing reactions, which differ in length by a single base pair. This necessitates using an acrylamide gel, usually at a concentration of 4–20%, of at least 40 cm in length and normally 0.4 mm thick.
KeywordsMigration Filtration Convection Urea Agarose
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