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Detection of BCR-ABL in Hematological Malignancies by RT-PCR

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Molecular Diagnosis of Cancer

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 6))

Abstract

The presence of a novel, minute chromosome m the cells of patients with chrome myeloid leukemia (CML) was first described m 1960 by Nowell and Hungerford (1). The Philadelphia (Ph) chromosome, as it became known, was shown subsequently by banding techniques to result from a recrprocal translocanon between the long arms of chromosomes 9 and 22 t(9;22) (q34;qll) (2). Molecular studies demonstrated that the translocatton disrupted the normal ABL and BCR genes on chromosomes 9 and 22, respectively, grvmg rise to a chimeric BCR-ABL gene encoding a fusion protein with transforming ability (3). The reciprocal ABL-BCR product is also transcriptionally active in the majority of cases (4, 5).

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Authors and Affiliations

  1. LRF Centre for Adult Leukaemia, Royal Postgraduate Medical School, London, UK

    Nicholas C. P. Cross

Authors
  1. Nicholas C. P. Cross
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Editor information

Editors and Affiliations

  1. University London, UK

    Finbarr E. Cotter

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© 1996 Humana Press Inc., Totowa, NJ

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Cross, N.C.P. (1996). Detection of BCR-ABL in Hematological Malignancies by RT-PCR. In: Cotter, F.E. (eds) Molecular Diagnosis of Cancer. Methods in Molecular Medicine™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-341-4:25

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  • DOI: https://doi.org/10.1385/0-89603-341-4:25

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-341-2

  • Online ISBN: 978-1-59259-590-7

  • eBook Packages: Springer Protocols

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