Abstract
The advent of recombinant DNA technology and the overexpression of heterologous proteins in bacteria have posed some unique problems not previously encountered in extraction of bacterial proteins. Some of this technology has enabled secretion of recombinant proteins by bacteria into the media, thereby eliminating the need to lyze the cells. However, most situations still require lysis of the bacterial cell wall in order to extract the recombinant protein product. A number of methods based on enzymatic and mechanical means are available for breaking open the bacterial cell wall, and the choice will depend on scale of process (1). Enzymatic methods use the activity of lysozyme, which cleaves the glucosidic linkages in the bacterial cell-wall polysaccharide. The inner cytoplasmic membrane can then be disrupted easily by detergents, osmotic pressure, or mechanical methods.
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© 1996 Humana Press Inc.
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Worrall, D.M. (1996). Extraction of Recombinant Protein from Bacteria. In: Doonan, S. (eds) Protein Purification Protocols. Methods in Molecular Biology™, vol 59. Humana Press. https://doi.org/10.1385/0-89603-336-8:31
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DOI: https://doi.org/10.1385/0-89603-336-8:31
Publisher Name: Humana Press
Print ISBN: 978-0-89603-336-8
Online ISBN: 978-1-59259-545-7
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