Abstract
The chapters that follow in this volume give detailed instructions on how to use the various methods that are available for purification of proteins. The question arises, however, of which of these methods to use and in which order to use them to achieve purification in any particular case. That is, the purification problem must be clearly defined. What follows outlines the sorts of questions that need to be asked as part of that definition and how the answers affect the approach that might be taken to developing a purification schedule. It should be noted here that the discussion does not touch on the special cases of purification of proteins at industrial scale or for therapeutic applications; these raise very specific problems that are outside the scope of this chapter (see refs. 1 and 2, respectively, for a coverage of these topics).
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Doonan, S. (1996). General Strategies. In: Doonan, S. (eds) Protein Purification Protocols. Methods in Molecular Biology™, vol 59. Humana Press. https://doi.org/10.1385/0-89603-336-8:1
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DOI: https://doi.org/10.1385/0-89603-336-8:1
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