Bulk Purification by Fractional Precipitation
The solubility of a particular protein in aqueous solution depends on the solvent composition and on the pH; hence, variation in these parameters provides a way of purifying proteins by fractional precipitation. The factors that dictate solubility are complex, since the surface of a protein is itself complex containing ionized residues, polar regions, and hydrophobic patches, all of which will interact with the solvent in ways that are not completely understood. Hence, it is not possible to elaborate a theoretical approach to fractional precipitation (1); rather, the methods are used in an essentially empirical fashion. The most widely used procedures are precipitation by addition of salt (salting out) and by addition of organic solvents; these are the focus of this chapter (see Note 1).
KeywordsProtein Solution Ammonium Sulfate Precipitate Protein Dialysis Tubing Fractional Precipitation
- 2.Dawson, R. M. C., Elliot, D. C., Elliot, W. H., and Jones, K. M. (1986) Data for Biochemical Research, 3rd ed. Oxford University Press, Oxford, UK, pp. 537–539.Google Scholar