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Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group- Specific Polymerase Chain Reaction

  • Frank van Kuppeveld
  • Jos van der Logt
  • Karl-Erik Johansson
  • Willem Melchers
Part of the Methods in Molecular Medicine book series (MIMM, volume 2)

Abstract

Cell cultures are widely used in both medical and biotechnical research centers, industries, and also as diagnostic tools in a clinical setting It has been reported that up to 50% of cell cultures are contaminated with mycoplasmas (1). Mycoplasma contamination may alter cellular growth characteristics, enzyme patterns, and cell membrane composition, and can induce chromosomal abnormalities and cytopathogenic changes (2, 3, 4, 5). In experimental results being published, it is now becoming standard practice to show that mycoplasma-free cell cultures have been used. For industrial production of biological materials derived from mammalian cells and intended for diagnostic or therapeutic use in humans, regulatory guidelines are emerging that are intended to guarantee the quality and safety of these products. Obligatory testing for mycoplasmas is therefore increasing (6)

Keywords

Polymerase Chain Reaction Sodium Dodecyl Sulfate Polymerase Chain Reaction Assay Oligonucleotide Probe Wash Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Frank van Kuppeveld
    • 1
  • Jos van der Logt
    • 1
  • Karl-Erik Johansson
    • 2
  • Willem Melchers
    • 2
  1. 1.Department of Medical MicrobiologyUniversity of NijmegenThe Netherlands
  2. 2.The National Veterinary InstituteUppsalaSweden

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