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Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta

  • Heide L. Kirschenlohr
  • James C. Metcalfe
  • David J. Grainger
Part of the Methods in Molecular Medicine book series (MIMM, volume 2)

Abstract

Abnormal proliferation of human vascular smooth muscle cells (hVSMCs) is a central event in the development of atherosclerosis (1, 2, 3) As a result, there is considerable interest in the establishment of hVSMC cultures as a mode1 of this disease process However, it has been noted in the past (4,5) that hVSMCs, especially when cultured by the enzyme-dispersal technique (hVSMC,ED), grow poorly in culture compared to VSMCs from other species (e.g., rat) This has limited their use for cell-culture studies. We have recently reported that the reduced proliferative capacity of hVSMCED from adult aorta can be attributed to the endogenous production of active TGF-β(6,7). We (6, 7, 8) and others (9, 10, 11) have shown that TGF-β; is a potent inhibtior of smooth muscle cell proliferation. Moreover, recent studies in animal models of atherosclerosis (12) have suggested that TGF-P plays a pivotal role in regulation of vessel wall architecture (13). We have also shown that hVSMCs derived by the alternative method of explanting (hVSMCEX) have a greater proliferative capacity than the hVSMCED (14) In accordance with our hypotheses, the cells grown from explanted tissue did not produce TGF-β; (14).

Keywords

Fetal Calf Serum Muscle Strip Scalpel Blade Plating Density Human Vascular Smooth Muscle Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Heide L. Kirschenlohr
    • 1
  • James C. Metcalfe
    • 1
  • David J. Grainger
    • 1
  1. 1.Department of BiochemistryUniversity of CambridgeUK

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