Abstract
It is often necessary to make multiple amino acid substitutions at a particular site to determine the function of the wild-type amino acid in protein structure and function studies. Each substitution requires a unique mutation at that site. An alternative to making a series of predetermined substitutions is to create a library of mutations at that site that encompasses all possible amino acid substitutions. The creation of such libraries is often simple. Screening the number of clones necessary to insure a complete representation of substitutions can be difficult and time consuming. Amino acid substitution by amber suppression provides an alternative to standard site-directed mutagenesis and library approaches (1).
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© 1996 Humana Press Inc.
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Lesley, S.A. (1996). Analysis of Point Mutations by Use of Amber Stop Codon Suppression. In: Trower, M.K. (eds) In Vitro Mutagenesis Protocols. Methods In Molecular Medicine™, vol 57. Humana Press. https://doi.org/10.1385/0-89603-332-5:65
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DOI: https://doi.org/10.1385/0-89603-332-5:65
Publisher Name: Humana Press
Print ISBN: 978-0-89603-332-0
Online ISBN: 978-1-59259-544-0
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