Abstract
The generation of nested deletions within cloned fragments of DNA has important applications in molecular biology. For DNA sequencing, nested deletions provide a series of overlapping templates that can be used to generate a composite sequence with a single sequencing primer; in gene and protein functional studies, nested deletions allow researchers to locate and define a variety of functional domains based on regions of deletions.
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© 1996 Humana Press Inc.
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Zhu, L., Holtz, A.E. (1996). A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site. In: Trower, M.K. (eds) In Vitro Mutagenesis Protocols. Methods In Molecular Medicine™, vol 57. Humana Press. https://doi.org/10.1385/0-89603-332-5:119
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DOI: https://doi.org/10.1385/0-89603-332-5:119
Publisher Name: Humana Press
Print ISBN: 978-0-89603-332-0
Online ISBN: 978-1-59259-544-0
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