A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site

  • Li Zhu
  • Ann E. Holtz
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 57)


The generation of nested deletions within cloned fragments of DNA has important applications in molecular biology. For DNA sequencing, nested deletions provide a series of overlapping templates that can be used to generate a composite sequence with a single sequencing primer; in gene and protein functional studies, nested deletions allow researchers to locate and define a variety of functional domains based on regions of deletions.


Restriction Enzyme Digestion Tn10 Insertion Unique Restriction Site Deletion Size Parental Plasmid 
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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Li Zhu
    • 1
  • Ann E. Holtz
    • 1
  1. 1.Clontech LaboratoriesPalo Alto

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