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Polymer-Supported Electrofusion of Protoplasts

A Novel Method and a Synergistic Effect

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Plant Cell Electroporation and Electrofusion Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 55))

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Abstract

Effective membrane electroporation is a direct and fast (in the millisecond range) field effect when an external electric field (EF) is applied. The applied field strength E induces the ionic interfacial transmembrane potential difference Δϕ m , which represents a contribution E m (Δϕ m ) (transmembrane field strength) to the mean EF force, causing structural rearrangements Δξ in the membrane phase. In brief, the electroporation is a sequence (1):

(1)

Theory guides the relationship:

(2)

where r is the cell radius and θ is the angle between membrane site and E vector. The conductivity factor f(κ) is an explicit function of the geometry of the cell and the conductivity of the solution, the membrane, and the cell interior.

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Author information

Author notes

  1. Lei Zhang

    Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA

Authors and Affiliations

  1. Laboratory of Bioelectrochemistry, Institute of Molecular Biotechnology, Jena, Germany

    Lei Zhang

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  1. Lei Zhang
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Editor information

Editors and Affiliations

  1. Harvard University, Boston, MA

    Jac A. Nickoloff

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© 1995 Humana Press Inc., Totowa, NJ

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Zhang, L. (1995). Polymer-Supported Electrofusion of Protoplasts. In: Nickoloff, J.A. (eds) Plant Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology™, vol 55. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-328-7:189

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  • DOI: https://doi.org/10.1385/0-89603-328-7:189

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-328-3

  • Online ISBN: 978-1-59259-542-6

  • eBook Packages: Springer Protocols

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