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Electrofusion of Plant Protoplasts

Selection and Screening for Somatic Hybrids of Nicotiana

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Plant Cell Electroporation and Electrofusion Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 55))

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Abstract

Discoveries in the 1960s and 1970s showed that plant protoplasts could be freed from their walls by digestion with fungal enzymes (1), grown in culture, and regenerated back into intact plants (2). This work opened the way for protoplast fusion and somatic hybridization in plants (3). In the ensuing 20 years, there have been substantial improvements in protoplast fusion and culture, and sophisticated approaches for the selection and characterization of somatic hybrids have been developed. As a result, in the 1990s, protoplast fusion has come of age, and fusion-derived somatic hybrids are being evaluated in practical plant breeding programs (4–6.

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Authors and Affiliations

  1. Department of Biological Sciences, Florida State University, Tallahassee, FL

    Harold N. Trick & George W. Bates

Authors
  1. Harold N. Trick
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  2. George W. Bates
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Editor information

Editors and Affiliations

  1. Harvard University, Boston, MA

    Jac A. Nickoloff

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© 1995 Humana Press Inc., Totowa, NJ

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Trick, H.N., Bates, G.W. (1995). Electrofusion of Plant Protoplasts. In: Nickoloff, J.A. (eds) Plant Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology™, vol 55. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-328-7:165

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  • DOI: https://doi.org/10.1385/0-89603-328-7:165

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-328-3

  • Online ISBN: 978-1-59259-542-6

  • eBook Packages: Springer Protocols

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