Pollen Electrotransformation in Tobacco

  • James A. Saunders
  • Benjamin F. Matthews
Part of the Methods in Molecular Biology™ book series (MIMB, volume 55)


Numerous techniques have been developed to transfer genes into plants to create genetically engineered crops that can tolerate environmental stresses, and to improve productivity and quality. The search for easier, more efficient techniques to transfer genes continues because the efficiencies of current techniques are low and recovering fertile transgenic plants is difficult and time consuming with some plant species.


Fluorescein Diacetate Trypan Blue Solution Tobacco Pollen Pollen Transformation Electroporation Condition 
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Copyright information

© Humana Press Inc., Totowa, NJ 1995

Authors and Affiliations

  • James A. Saunders
    • 1
  • Benjamin F. Matthews
    • 2
  1. 1.Soybean and Alfalfa Research Laboratory, Plant Sciences InstituteUSDA-ARSBeltsville
  2. 2.Plant Molecular Biology LaboratoryUSDA-ARSBeltsville

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