The Identification of Dengue Virus Using PCR
Part of the Methods in Molecular Biology™ book series (MIMB, volume 50)
Dengue virus is a member of the family Flaviviridae with a single-stranded positive sense RNA genome. The virus particle consists of a lipid bilayer with envelope (E) and membrane (M) proteins surrounding a spherical nucleocapsid composed of a genome RNA and capsid (C) proteins. The virion structure is easily destroyed by treatment with non-ionic detergent (see Fig. 1) or low osmotic shock. Four antigenically related but distinct serotypes of dengue virus (DEN1, DEN2, DEN3, and DEN4) are circulating in tropical countries and cause dengue fever and dengue hemorrhagic fever, both of which are highly prevalent.
KeywordsVortex Sucrose Agarose Bromide Heparin
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© Humana Press Inc. 1996