Abstract
Agrobacterium-mediated transfer of cloned DNA copies of infectious viral genomes to plant tissues has been termed “agroinoculation” or “agroinfection” (for review, see ref. 1). The technique has been used successfully for a wide variety of virus groups, including the caulimoviruses and badnaviruses (genomes of double-stranded [ds] DNA) (2–4) the geminiviruses (ssDNA genomes) (5–7), the phloem-restricted luteoviruses (ssRNA genomes) (8), as well as for viroids (ssRNA) (9). For viruses with RNA genomes and viroids, cloned cDNA copies of the viral genome are required. Agroinfection of viruses (or viroids) with circular genomes requires the insertion of tandem multimeric copies of the genome between the T-DNA borders of an Agrobacterium binary vector, but for RNA viruses with linear genomes a cDNA copy of the genome must be inserted between a promoter (usually, the cauliflower mosaic virus 35S promoter [CaMV 35S]) and a transcription termination signal (e.g., the nopaline synthase terminator) (8).
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References
Grimsley, N (1990) Agroinfection. Physiologia Plantarum 79, 147–153.
Grimsley, N. H., Hohn, B., Hohn, T., and Walden, R. (1986) Agroinfection, an alternative route for viral infection of plants by using the Ti plasmid. Proc. Natl. Acad. Sci. USA 83, 3282–3286.
Dasgupta, I., Hull, R., Eastop, S., Poggi-Pollini, C., Blakebrough, M., Boulton, M. I., and Davies, J. W. (1991) Rice tungro bacilliform virus DNA independently infects rice after Agrobacterium-mediated transfer. J. Gen. Virol. 72, 1215–1221.
Medbury, S. L., Lockhart, B. E. L., and Olszewski, N. E. (1991) Properties of Commelina mottle virus’s complete DNA sequence, genomic discontinuities and transcript suggest that it is a pararetrovirus. Nucleic Acids Res. 18, 5505–5513.
Grimsley, N. H., Hohn, T., Davies, J. W., and Hohn, B. (1987) Agrobacterium-mediated delivery of maize streak virus into maize plants. Nature 325, 177–179.
Hayes, R. J., Coutts, R. H. A., and Buck, K. W. (1988) Agroinfection of Nicotiana spp. with cloned DNA of tomato golden mosaic virus. J. Gen. Virol 69, 1487–1496.
Briddon, R. W., Watts, J., Markham, P. G., and Stanley, J. (1989) The coat protein of beet curly top virus is essential for infectivity. Virologv 172, 628–633.
Leiser, R.-M., Ziegler-Graff, V., Reutenauer, A., Herrbach, E., Lemaire, O., Guilley, H., Richards, K., and Jonard, G. (1992) Agroinfection as an alternative to insects for infecting plants with beet western yellows luteovirus. Proc. Nutl. Acad Sci. USA. 89, 9136–9140.
Gardner, R., Chonoes, K., and Owens, R. (1986) Potato spindle tuber viroid infections mediated by the Ti plasmid of Agrobacterium tumefaciens. Plant Mol. Biol. 6, 221–228.
Stenger, D. C., Revington, G. N., Stevenson, M. C., and Bisaro, D. M. (1991) Replicational release of geminivirus genomes from tandemly-repeated copies: evidence for rolling circle replication of a plant viral DNA. Proc. Natl. Acad Sci. USA 88, 8029–8033.
Boulton, M. I., Buchholz, W. G., Marks, M. S., Markham, P. G., and Davies, J. W. (1989) Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. Plant Mol. Biol 12, 31–40.
Boulton, M. I., Ramerr, D. M., Davies, J. W., and Nester, E. W. (1993) Identification of genetic factors controlling the ability of Agrobacterium to transfer DNA to maize, in Advances in Molecular Genetics of Plant-Microbe Interactions (Nester, E. W. and Verma, D. P. S., eds.), Kluwer, The Netherlands, pp 73–78.
Grimsley, N., Jarchow, E., Oetiker, J., Schlaeppi, M., and Hohn, B. (1991) Agroinfection as a tool for the investigation of plant-pathogen interactions, in Plant Molecular Biology 2 (Herrmann, R. G. and Larkins, B., eds.), Plenum, New York, pp. 225–238.
Marks, M. S., Kemp, J. M., Woolston, C. J., and Dale, P. J. (1989) Agroinfection of wheat: a comparison of Agrobacterium strains. Plant Sci. 63, 247–256.
Davies, J. W. and Stanley, J. (1989) Geminivirus genes and vectors. Trends. Genet 5, 77–81.
Lazarowitz, S. G. (1987) The molecular characterization of geminrviruses. Plant Mol. Biol. Rept. 4, 177–192.
Lazarowitz, S. G. (1992) Geminiviruses: genome structure and gene function. Crit. Rev. Plant Sci. 11, 327–349.
von Arnim, A., Frischmuth, T., and Stanley, J. (1993) Detection and possible functions of African lease cassava mosaic virus DNA B gene products. Virology 192, 264–272.
De Cleene, M. and DeLey, G. (1976) The host range of crown gall. Bot. Rev. 42, 389–466.
De Cleene, M. (1984) The susceptibility of monocotyledons to Agrobacterium tumefaciens. Phytopath Z 113, 81–
Boulton, M. I. and Davies, J. W. (1990) Monopartite geminiviruses: markers for gene transfer to cereals. Aspects of Appl. Biol 24, 79–86.
Raineri, D. M., Boulton, M. I., Davies, J. W., and Nester, E. W. (1993) VirA, the plant-signal receptor, is responsible for the Ti plasmid-specific transfer of DNA to maize by Agrobacterium. Proc. Natl. Acad. Sci. USA 90, 3549–3553.
Stanley, J. (1991) The molecular determinants of geminivirus pathogenesis. Semin Virol 2, 139–149.
Briddon, R. W., Pinner, M. S., Stanley, J., and Markham, P. G. (1990) Geminivirus coat protein gene replacement alters insect specificity. Virology 177, 85–94.
Etessami, P., Callis, R., Ellwood, S., and Stanley, J. (1988) Delimitation of essential genes of cassava latent virus DNA 2. Nucleic Acids Res. 16, 4811–4829.
von Arnim, A. and Stanley, J. (1992) Inhibition of African cassava mosaic virus systemic infection by a movement protein from the related geminivirus tomato golden mosaic virus. Virology 187, 555–564.
Sunter, G. and Bisaro, D. (1992) Transactivation of geminivirus AR1 and BR1 gene expression by the viral AL2 gene product occurs at the level of transcription. Plant Cell 4, 1321–1331.
Stanley, J. and Townsend, R. (1986) Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA clones containing single copies of the genome. Nucleic Acids Res. 14, 5981–5998.
Brough, C. L., Hayes, R. J., Morgan, A. J., Coutts, R. H. A., and Buck, K. W. (1988) Effects of mutagenesis in vitro on the ability of cloned tomato golden mosaic virus DNA to infect Nicotiana benthamiana plants. J. Gen. Virol 69, 503–514.
Ward, A., Etessami, P., and Stanley, J. (1988) Expression of a bacterial gene in plants mediated by infectious geminivirus DNA. EMBO J. 7, 1583–1587.
Hayes, R. J., Petty, I. T. D., Coutts, R. H. A., and Buck, K. W. (1988) Gene amplification and expression in plants by a replicating geminivirus vector. Nature 334, 179–182.
Hayes, R. J., Coutts R. H. A., and Buck, K. W. (1989) Stability and expression of bacterial genes in replicating geminivirus vectors in plants. Nucleic Acids Res. 17, 2391–2403.
Elmer, S. and Rogers, S. G. (1990) Selection for wild type derivatives of tomato golden mosaic virus during systemic infection. Nucleic Acids Res. 18, 2001–2006.
Hanley-Bowdoin, L., Elmer, J. S., and Rogers, S. G. (1988) Transient expression of heterologous RNAs using tomato golden mosaic virus. Nucleic Acids Res. 16, 10,511–10,528.
Boulton, M. I., Steinkellner, H., Donson, J., Markham, P. G., King, D. I., and Davies, J. W. (1989) Mutational analysis of the virion-sense genes of maize streak virus. J. Gen. Virol. 70, 2309–2323.
Woolston, C. J., Reynolds, H. V., Stacey, N. J., and Mullineaux, P. M. (1989) Replication of wheat dwarf virus DNA in protoplasts and analysis of coat protein mutants in protoplasts and plants. Nucleic Acids Res. 17, 6029–6041.
Shen, W. H. and Hohn, B. (1994) Amplification and expression of the β-glucuronidase gene in maize plants by vectors based on maize streak virus. Plant J., 5, 227–236.
Shen, W.-H. and Hohn, B. (1992) Excision of a transposable element from a viral vector introduced into maize plants by agroinfection. Plant J. 2, 35–42.
Shen, W.-H, Das, S., and Hohn, B. (1992) Mechanism of DsI excision from the genome of maize streak virus. Mol. Gen. Genet. 233, 388–394.
McCullough, A. J., Lou, H., and Schuler, M. A. (1991) In vivo analysis of plant pre-mRNA splicing using an autonomously replicating vector. Nucleic Acids Res. 19, 3001–3009.
Mullineaux, P. M., Donson, J., Morris-Krsinich, B. A. M., Boulton, M. I., and Davies, J. W. (1984) The nucleotide sequence of maize streak virus DNA. EMBO J. 3, 3063–3068.
Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12, 8711–8721.
Zambryski, P. J., Joos, H., Leemans, J., Van Montagu, M., and Schell, J. (1983) Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J. 2, 2143–2150.
Cangelosi, G. A., Best, E. A., Martinetti, G., and Nester, E. W. (1991) Genetic analysis of Agrobacterium. Methods Enzymol 204, 384–397.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Montoya, A. L., Chilton, M.-D., Gordon, M. P., Sciaky, D., and Nester, E. W. (1977) Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes. J. Bacteriol. 129, 101–107.
Murant, A. F. and Harrison, B D. (eds.) CMI/AAB Descriptions of Plant Viruses. Sets 1–19 (1970–1989) Association of Applied Biologists, Warwick, UK.
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© 1995 Humana Press Inc., Totowa, NJ
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Boulton, M.I. (1995). Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues. In: Jones, H. (eds) Plant Gene Transfer and Expression Protocols. Methods in Molecular Biology™, vol 49. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-321-X:77
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DOI: https://doi.org/10.1385/0-89603-321-X:77
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