Abstract
A number of methods have been developed for the isolation of plant genes, including the differential screening of cDNA libraries, the use of heterologous probes, and the use of protein sequence data to generate oligonucleotides for library screening. These have been well-reviewed. The advent of plant transformation techniques has further increased the tools available for the functional analysis of isolated genes, such as for the introduction of promoter deletion-reporter gene fusions, for the overexpression of the gene product or for generating null expressors by antisense RNA technology. More recently, transformation techniques have been exploited to develop new approaches to isolate plant genes, and these methods can be considered as being of two types: insertional mutagenesis and gene trapping.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dean, C., Sjodin, C., Page, T., Jones, J., and Lister, C. (1992) Behaviour of the maize transposable element AC in Arabidopsis thaliana. Plant J. 2, 69–81.
Topping, J. F., Wei, W., and Lindsey, K. (1991) Functional tagging of regulatory elements in the plant genome. Development 112, 1009–1019.
Goldsbrough, A. and Bevan, M. (1991) New patterns of gene activity in plants detected using an Agrobacterium vector. Plant Mol. Biol. 16, 263–269.
Lindsey, K., Wei, W., Clarke, M. C., McArdle, H. F., Rooke, L. M., and Topping, J. F. (1993) Tagging genomic sequences that direct transgene expression by activation of a promoter trap in plants. Transgenic Research 2, 33–47.
André, D., Colau, D., Schell, J., van Montagu, M., and Hernalsteens, J.-P. (1986) Gene tagging in plants by a T-DNA insertion mutagen that generates APH (3′)II-plant gene fusions. Mol. Gen. Genet. 204, 512–518.
Koncz, C., Martini, N., Mayerhofer, R., Koncz-Kalman, Z., Körber, H., Rédei, G. P., and Schell, J. (1989) High frequency T-DNA-mediated gene tagging in plants. Proc. Natl. Acad. Sci. USA 86, 8467–8471.
Kertbundit, S., De Greve, H., DeBoeck, F., van Montagu, M., and Hernalsteens, J.-P. (1991) In vivo random β-glucuronidase gene fusions in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 88, 5212–5216.
Yanofsky, M. F., Ma, H., Bowman, J. L., Drews, G. N., Feldmann, K. A., and Meyerowitz, E. M. (1990) The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature 346, 35–39.
Koncz, C., Mayerhofer, R., Koncz-Kalman, Z., Nawrath, C., Reiss, B., Rédei, G. P., and Schell, J. (1990) Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana. EMBO J. 9, 1337–1346.
Rédei, G. P. (1975) Arabidopsis as a genetic tool. Ann. Rev. Genet. 9, 111–127.
Rédei, G. P. and Koncz, C. (1992) Classical mutagenesis, in Methods in Arabidopsis Research (Koncz, C., Chua, N. H., and Schell, J., eds.), World Scientific, Singapore, pp. 16–82.
Meyerowitz, E. M. (1989) Arabidopsis, a useful weed. Cell 56, 263–269.
Tsay, Y.-F., Frank, M. J., Page, T., Dean, C., and Crawford, N. M. (1993) Identification of a mobile endogenous transposon in Arabidopsis thaliana. Science 260, 342–344.
Lindsey, K. and Jones, M. G. K. (1990) Selection of transformed plant cells, in Plant Cell Line Selection: Procedures and Applications (Dix, P. J., ed.), VCH Verlagsgesellschaft, Weinheim, pp. 317–339.
Damm, B. and Willmitzer, L. (1991) Arabidopsis protoplast transformation and regeneration, in Plant Tissue Culture Manual: Fundamentals and Applications (Lindsey, K., ed.), Kluwer, Dordrecht, pp. A7: 1–20.
Feldmann, K. A. and Marks, M. D. (1987) Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: a non-tissue culture approach. Mol. Gen. Genet. 208, 1–9.
Schmidt, R. and Willmitzer, L. (1991) Arabidopsis regeneration and transformation (leaf and cotyledon explant system), in Plant Tissue Culture Manual Fundamentals and Applications (Lindsey, K, ed.), Kluwer, Dordrecht, pp. A6: 1–17.
Valvekens, D., van Lijsebettens, M., and van Montagu, M. (1992) Arabidopsis regeneration and transformation (root explant system), in Plant Tissue Culture Manual Fundamentals and Applications (Lindsey, K, ed.), Kluwer, Dordrecht, pp. A8 1–17.
Forsthoefel, N. R., Wu, Y., Schulz, B., Bennett, M. J., and Feldmann, K. A. (1992) T-DNA insertion mutagenesis in Arabidopsis. prospects and perspectives. Aust. J Plant Physiol. 19, 353–366.
Clarke, M. C., Wet, W., and Lindsey, K. (1992) High frequency transformation of Arabidopsis thaliana by Agrobacterium tumefaciens. Plant Mol. Biol Reporter 10, 178–189.
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15, 473–497.
Gamborg, O. L., Miller, R. A., and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158.
Russell, J., Fuller, J., Wilson, Z., and Mulligan, B. (1991) Protocol for growing Arabidopsis, in Arabidopsis The Compleat Guide (Flanders, D. and Dean, C., eds.), AFRC PMB Arabidopsis Programme, Norwich, UK.
Jefferson, R. A, Kavanagh, T. A., and Bevan, M. W. (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901–3907.
Topping, J. F., Agyeman, F., Henricot, B., and Lindsey, K. (1994) Identification of molecular markers of embryogenesis in Arabidopsis thaliana by promoter trapping. Plant J. 5, 895–903.
Collins, F. S., and Weissman, S. M. (1984) Directional cloning of DNA fragments at a large distance from an initial probe: a circularized method. Proc. Natl. Acad. Sci. USA 81, 6812–6816.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Topping, J.F., Wei, W., Clarke, M.C., Muskett, P., Lindsey, K. (1995). Agrobacterium-Mediated Transformation of Arabidopsis thaliana. In: Jones, H. (eds) Plant Gene Transfer and Expression Protocols. Methods in Molecular Biology™, vol 49. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-321-X:63
Download citation
DOI: https://doi.org/10.1385/0-89603-321-X:63
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-321-4
Online ISBN: 978-1-59259-536-5
eBook Packages: Springer Protocols