Abstract
The complex nature of plant metabolism often can seriously hinder the functional analysis of many proteins; and this has led some researchers to attempt to express such proteins in less sophisticated organisms. Unfortunately, the obvious choice, Escherichia coli, is less than ideal with many foreign peptides being misfolded and subsequently sequestered in insoluble protein bodies. The yeast Saccharomyces cerevisiae, however, has a combination of properties that make it a particularly attractive system for the functional expression of plant proteins.
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© 1995 Humana Press Inc., Totowa, NJ
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Trueman, L.J. (1995). Heterologous Expression in Yeast. In: Jones, H. (eds) Plant Gene Transfer and Expression Protocols. Methods in Molecular Biology™, vol 49. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-321-X:341
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DOI: https://doi.org/10.1385/0-89603-321-X:341
Publisher Name: Springer, Totowa, NJ
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