Abstract
The cereals are one of the groups of crops more recalcitrant to transformation. Since, with the exception of rice (1), cereals have not been transformed by Agrobacterium, and highly regenerable protoplast systems are difficult to obtain, these species have remained untransformed for a much longer time than the major dicot species. It was not until 1988 and 1989 that the first transgenic cereal plants (maize [2]; rice [3]) were produced by the use of direct DNA-transfer into protoplasts. This method, however, has not yet given rise to transgenic wheat or barley plants, species in which the protoplast systems established are not as regenerative and stable as those developed for rice or maize.
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References
Hiei, Y., Ohta, S., Komari, T., and Kumashiro, T. (1994) Efficient transformation of rice (Oryza sativa L) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J. 6, 271–282.
Rhodes, C. A., Pierce, D. A., Mettler I. J., Mascarenhas, D., and Detmer, J. J. (1988) Genetically transformed maize plants from protoplasts. Science 240, 204–207.
Shimamoto, K., Terada, R., Izawa, T., and Fujimoto, H. (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338, 274–276.
Sanford, J. C., Klein, T. M., Wolf, E. D., and Allen, N. (1987) Delivery of substances into cells and tissues using a particle bombardment process. J. Part. Sci Technol. 5, 27–37.
Gordon-Kamm, W. J., Spencer, T. M., Mangano, M. L, Adams, T. R., Daines, R J., Start, W. G., OBrien, J. V., Chambers, S. A., Adams, W. R., Jr., Willets, N. G., Rice, T. B., Mackey, C. J., Krueger, R. W, Kausch, A. P., and Lemaux, P. G. (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2, 603–618.
Cao, J., Duan, X., McElroy, D., and Wu, R. (1992) Regeneration of herbicide resistant transgenic race plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Reps. 11, 586–591.
Vasil, V., Castillo, A. M., Fromm, M. E., and Vasil, I. K. (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10, 667–674.
Christou, P., Ford, T. L., and Kofron, M. (1991) Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9, 957–962.
Koziel, M. G., Beland, G. L., Bowman, C., Carozzi, N. B., Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M., Kadwell, S., Launis, K., Lewis, K., Maddox, D., McPherson, K., Meghji, M. R., Merlin, E., Rhodes, R., Warren, G. W., Wright, M., and Evola, S. V. (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technology 11, 194–200.
Weeks, T., Anderson, O. D., and Blechl, A. E. (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102, 1077–1084.
Vasil, V., Srivastava, V., Castillo, A. M., Fromm, M. E., and Vasil, I. K. (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11, 1553–1558.
Becker, D., Brettschneider, R., and Lorz, H. (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J. 5, 299–307.
Nehra, N. S., Chibbar, R. N., Leung, N., Caswell, K., Mallard, C., Steinhauer, L., Baga, M., and Kartha, K. K. (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J. 5, 285–297.
Wan, Y. and Lemaux, P. G. (1994) Generation of large numbers of independently transformed fertile barley plants. Plant Physiol. 104, 37–48.
Barcelo, P., Vazquez, A., and Martin, A. (1989) Somatic embryogenesis and plant regeneration from tritordeum. Plant Breeding 103, 235–240.
Barcelo, P., Hagel, C., Becker, D., Martin, A., and Lorz, H. (1994) Transgenic cereal (tritordeum) plants obtained at high efficiency by microprojectile bombardment of inflorescence tissue. Plant J. 5, 583–592.
Martin, A. and Sanchez-Monge, E. (1982) Cytology and morphology of the amphiploid Hordeum chilense x Triticum turgidum conv. durum Euphytica 31, 261–267.
Lazzeri, P. A. and Shewry, P. R. (1993) Biotechnology of cereals, in Biotechnology and Genetic Engineering Reviews, vol. 11 (Tombs M. P., ed.), Intercept Ltd., Andover, MD, pp. 79–145.
McElroy, D., Zhang, W., Cao, J., and Wu, R. (1990) Isolation of an efficient Actin promoter for use in rice transformation. Plant Cell 2, 163–171.
Callis, J., Fromm, M., and Walbot, V. (1987) Introns increase gene expression in cultured maize cells. Genes Dev. 1, 1183–1200.
Jefferson, R. A. (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 5, 387–405.
Schreier, P. H., Seftot, E. A., Schell, J., and Bohnert, H. J. (1985) The use of nuclear encoded sequences to detect the light regulated synthesis and transport of a foreign protein into plant chloroplast. EMBO J. 4, 25–32.
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© 1995 Humana Press Inc., Totowa, NJ
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Barcelo, P., Lazzeri, P.A. (1995). Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues. In: Jones, H. (eds) Plant Gene Transfer and Expression Protocols. Methods in Molecular Biology™, vol 49. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-321-X:113
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DOI: https://doi.org/10.1385/0-89603-321-X:113
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