Heterologous Expression in Yeast
The complex nature of plant metabolism often can seriously hinder the functional analysis of many proteins; and this has led some researchers to attempt to express such proteins in less sophisticated organisms. Unfortunately, the obvious choice, Escherichia coli, is less than ideal with many foreign peptides being misfolded and subsequently sequestered in insoluble protein bodies. The yeast Saccharomyces cerevisiae, however, has a combination of properties that make it a particularly attractive system for the functional expression of plant proteins.
KeywordsGlycerol Phenol DMSO Glycol Fractionation
- 3.Riesmeier, J., Willmitzer, L., and Frommer, W. (1992) Isolation and characterisation of a sucrose carrier cDNA from spinach by functional expression in yeast. EMBO 11, 4705–4713.Google Scholar
- 13.Lorincz, A. (1984) Quick preparation of plasmid DNA from yeast. Focus 6, 11.Google Scholar
- 14.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar