Heterologous Expression in Yeast

  • Laurence J. Trueman
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 49)

Abstract

The complex nature of plant metabolism often can seriously hinder the functional analysis of many proteins; and this has led some researchers to attempt to express such proteins in less sophisticated organisms. Unfortunately, the obvious choice, Escherichia coli, is less than ideal with many foreign peptides being misfolded and subsequently sequestered in insoluble protein bodies. The yeast Saccharomyces cerevisiae, however, has a combination of properties that make it a particularly attractive system for the functional expression of plant proteins.

Keywords

Glycerol Phenol DMSO Glycol Fractionation 

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Copyright information

© Humana Press Inc., Totowa, NJ 1995

Authors and Affiliations

  • Laurence J. Trueman
    • 1
  1. 1.IACR-Rothamsted Experimental StationHarpendenUK

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