Abstract
Wild-type and industrial strains of Saccharomyces cerevisiae appear to be typically polyploid/aneuploid. Such strains are also essentially sterile, but will on rare occasions produce spores. The mating of such spores with reference laboratory strains can be used to give a general estimation of the overall ploidy level. Similarly, measurement of a parameter that is proportionally maintained relative to ploidy level in a euploid reference series, such as DNA content or a certain enzyme activity, can be used to estimate the general overall ploidy of wild-type or industrial strains (1,2). However, this does not take account of interstrain variability and, in particular, aneuploidy.
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© 1996 Humana Press Inc., Totowa, NJ
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Hadfield, C., Jordan, B.E., Mount, R.C. (1996). Determination of Chromosome Ploidy in Yeast. In: Evans, I.H. (eds) Yeast Protocols. Methods in Molecular Biology™, vol 53. Humana Press. https://doi.org/10.1385/0-89603-319-8:205
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DOI: https://doi.org/10.1385/0-89603-319-8:205
Publisher Name: Humana Press
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