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Method Development/Optimization

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Book cover Capillary Electrophoresis Guidebook

Part of the book series: Methods in Molecular Biology ((MIMB,volume 52))

Abstract

This chapter provides some possible starting points for method development and further optimization. A later subsection in this chapter presents some starting points and guidelines for method development of chiral separations. A more extensive treatment of chiral separations is given in Chapter 14. A detailed background to the parameters affecting MECC separations is given in Chapter 12.

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References

  1. McLaughlin, G. M., Nolan, J. A., Lindahl, J. L., Morrison, J. A., and Bronzert, T. J. (1992) Practical drug separations by HPCE: practical considerations. J. Liquid Chromatogr. 15, 961–1021.

    Article  CAS  Google Scholar 

  2. Yeo, S. K., Ong, C. P., and Li, S. F. Y. (1991) Optimisation of high-performance capillary electrophoresis of plant growth regulators using the overlapping resolution mapping scheme. Anal. Chem. 63, 2222–2225.

    Article  CAS  Google Scholar 

  3. Chadwick, R. K. and Hsieh, J. C. (1991) Separation of cis and trans double bond isomers using capillary zone electrophoresis. Anal. Chem. 63, 2377–2380.

    Article  CAS  Google Scholar 

  4. Weinmann, W., Maier, C., Baumeister, K., Przybylski, M., Parker, C. E., and Tomer K. B. (1994) Isolation of hydrophobic lipoproteins in organic solvents by pressure assisted capillary electrophoresis for subsequent mass spectrometric characterization. J. Chromatogr. 664, 271–275.

    Article  CAS  Google Scholar 

  5. Sciacchitano, C. J., Mopper, B., and Specchio, J. J. (1994) Identification and separation of five cephalosporins by micellar electrokinetic capillary chromatography. J. Chromatogr. 657, 395–399.

    Article  CAS  Google Scholar 

  6. Rush, R. S., Derby, P. L., Strickland, T. W., and Rhode, F. (1993) Peptide mapping and evaluation of glycopeptide microheterogeneity derived from endoproteinase digestion of erythropoietin by affinity high-performance capillary electrophoresis. Anal. Chem. 65, 1834–1842.

    Article  CAS  Google Scholar 

  7. Nesi, M., Chiari, M., Carrea, G., Ottolina, G., and Righetti, P. G. (1994) Capillary electrophoresis of nicotinamide-adenine dinucleotide and nicotinamide-adenine dinucleotide phosphate derivatives in coated tubular columns. J. Chromatogr. 670, 215–221.

    Article  CAS  Google Scholar 

  8. Atamna, I. Z., Metral, C. J., Muschik, G. M., and Issaq, J. (1990) Factors which influence the mobility, resolution and selectivity in capillary zone electrophoresis III The role of the buffer cation. J. Liquid Chromatogr. 13, 3201–3210.

    Article  CAS  Google Scholar 

  9. Bushey, M. M. and Jorgenson, J. W. (1989) Capillary electrophoresis of proteins in buffers containing high concentrations of zwitterionic salts. J. Chromatogr. 480, 301–310.

    Article  CAS  Google Scholar 

  10. Altria, K. D., Goodall D. M., and Rogan, M. M. (1994) Quantitative determination of drug counter-ion stoichiometry by capillary electrophoresis. Chromatographia 38, 637–642.

    Article  CAS  Google Scholar 

  11. Liu, Y.-M. and Sheu, S.-J. (1994) Separation of aromatic acids by reversed electroosmotic flow capillary electrophoresis. J. Chromatogr. 663, 239–243.

    Article  CAS  Google Scholar 

  12. Honda, S., Taga, A., Kakehi, K., Koda, S., and Okamoto, Y. (1992) Determination of cefixime and its metabolites by high-performance capillary electrophoresis. J. Chromatogr. 590, 364–368.

    Article  CAS  Google Scholar 

  13. Bullock, J. A. and Yuan, L.-C. (1991) Free solution capillary elecrophoresis of basic proteins in uncoated fused-silica capillary tubing. J. Micro. Sep. 3, 241–248.

    Article  CAS  Google Scholar 

  14. Foly, J. P. (1990) Optimisation of micellar electrokinetic chromatography. Anal. Chem. 62, 1302–1308.

    Article  Google Scholar 

  15. Strasters, J. K. and Khaledi, M. G. (1991) Migration behaviour of cationic solutes in micellar electrokinetic capillary chromatography. Anal. Chem. 63, 2502–2508.

    Article  Google Scholar 

  16. Cole, R. O., Sepaniak, M. J., Hinze, W. L., Gorse, J., and Oldiges, K. (1991) Bile salt structures in micellar electrokinetic capillary chromatography. Application to hydrophobic molecule separations. J. Chromatogr. 557, 113–123.

    Article  CAS  Google Scholar 

  17. Ingvardsen, L., Michaelsen, S., and Sorensen, H. (1994) Analysis of individual phospholipids by high performance capillary electrophoresis. J. Am. Oil Chem. Soc. 71, 183–188.

    Article  CAS  Google Scholar 

  18. Crosby, D. and El Rassi, Z. (1993) Micellar electrokinetic capillary chromatography with cationic surfactants. J. Liquid Chromatogr. 16, 2161–2187.

    Article  CAS  Google Scholar 

  19. Goebel, L. K. and McNair, H. M. (1991) Optimisation of resolution in micellar electrokinetic chromatography. JHRCC 14, 25.

    Google Scholar 

  20. Terabe, S., Ishihama, Y., Nishi, H., Fukuyama, T., and Otsuka, K. (1991) Effect of urea addition in micellar electrokinetic chromatography. J. Chromatogr. 545, 359–368.

    Article  CAS  Google Scholar 

  21. Wehr, T. (1993) Recent advances in capillary electrophoresis columns. LC-CG 11, 14–20.

    CAS  Google Scholar 

  22. Hjerten, S. (1993) Electrophoresis 14, 390.

    Article  CAS  Google Scholar 

  23. Smith, J. T. and Rassi, El (1993) Electrophoresis 14, 396.

    Article  CAS  Google Scholar 

  24. Chen, M. and Cassidy, R. M. (1992) Bonded-phase capillaries and the separation of inorganic ions by capillary zone electrophoresis. J. Chromatogr. 602, 227–234.

    Article  CAS  Google Scholar 

  25. Dougherty, A. M., Wooley, C. L., Williams, D. L., Swaile, D. F., Cole, R. O., and Sepaniak, M. J. (1991) Stable phases for capillary electrophoresis. J. Liquid Chromatogr. 14, 907–912.

    Article  CAS  Google Scholar 

  26. Cobb, K. A., Dolnik, V., and Novotony, M. (1990) Electrophoresis of proteins in capillaries with hydrolytically stable surface structures. Anal. Chem. 62, 2478–2483.

    Article  CAS  Google Scholar 

  27. Hoffstetter-Kuhn, S., Paulus, A., Gassmann, E., and Widmer, H. M. (1991) Influence of borate complexation on the electrophoretic behaviour of carbohydrates in capillary electrophoresis. Anal. Chem. 63, 1541–1547.

    Article  CAS  Google Scholar 

  28. Ackermans, M. T., Everaerts, F. M., and Beckers, J. L. (1991) Determination of some drugs by micellar electrokinetic capillary chromatography. The pseudo-effective mobility as parameter for screening. J. Chromatogr. 585, 123–131.

    Article  CAS  Google Scholar 

  29. Altria, K. D. and Chanter, Y. (1993) Validation of a capillary electrophoresis method for the determination of a quinoline antibiotic and its related impurities. J. Chromatogr. 652, 459–463.

    Article  CAS  Google Scholar 

  30. Coufal, P., Stulik, K., Claessens, H. A., and Cramers, C. A (1994) The magnitude and reproducibility of the electroosmotic flow in silica capillary tubes. JHRCC 17, 325–334.

    CAS  Google Scholar 

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© 1996 Humana Press Inc., Totowa, NJ

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Altria, K.D. (1996). Method Development/Optimization. In: Altria, K.D. (eds) Capillary Electrophoresis Guidebook. Methods in Molecular Biology, vol 52. Humana Press. https://doi.org/10.1385/0-89603-315-5:29

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  • DOI: https://doi.org/10.1385/0-89603-315-5:29

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-315-3

  • Online ISBN: 978-1-59259-539-6

  • eBook Packages: Springer Protocols

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