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Part of the book series: Methods in Molecular Biology ((MIMB,volume 52))

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Abstract

This chapter covers the possible solutions to problems that may be encountered in routine operation. Further details may be available in specific instrument manuals or by contacting appropriate service specialists. The most likely problem areas are:

  1. 1.

    Voltage/current problems—Chart 1.

  2. 2.

    No peaks obtained—Chart 2.

  3. 3.

    Poor peak shape/height—Chart 3.

  4. 4.

    Poor peak time precision—Chart 4.

  5. 5.

    Poor peak area precision—Chart 5.

  6. 6.

    Poor sensitivity—Chart 6.

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References

  1. Ackermans, M. T., Everaerts, F. M., and Beckers, J. L. (1991) Determination of some drugs by micellar electrokinetic capillary chromatography. The pseudo-effective mobility as parameter for screening. J. Chromatogr. 585, 123–131.

    Article  CAS  Google Scholar 

  2. Shafaati, A., and Clark, B. J. (1993) Development of a capillary zone electrophoresis method for atenolol and its related impurities in a tablet preparation. Anal. Proc. 30,481–483.

    CAS  Google Scholar 

  3. Zhu, T., Sun, Y.-L., Zhang, C.-X., Ling, D.-K., and Sun, Z.-P. (1994) Variation of pH of the background electrolyte as a result of electrolysis in CE. JHRCC 17, 563,564.

    CAS  Google Scholar 

  4. Thomas, B. R., Fang, X. G., Chen, X., Tyrell, R. J., and Ghodbane, S. (1994) Validated micellar electrokinetic capillary chromatography method for the quality control of the drug substances hydrochlorothiazide and chlorothiazide. J. Chromatogr. 657, 383–394.

    Article  CAS  Google Scholar 

  5. Altria, K. D. (1993) Essential peak area normalisation in capillary electrophoresis Chromatographia 35, 177–182.

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© 1996 Humana Press Inc., Totowa, NJ

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Altria, K.D. (1996). Troubleshooting. In: Altria, K.D. (eds) Capillary Electrophoresis Guidebook. Methods in Molecular Biology, vol 52. Humana Press. https://doi.org/10.1385/0-89603-315-5:105

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  • DOI: https://doi.org/10.1385/0-89603-315-5:105

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-315-3

  • Online ISBN: 978-1-59259-539-6

  • eBook Packages: Springer Protocols

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