Abstract
There are many methods describing the preparation of DNA from Saccharomyces cerevisiae. The method described in this chapter is a modification of those described by Olson et al. (1) and Philippsen et al. (2). Although simpler techniques are available (3), this method routinely provides relatively high molecular weight DNA (50–250 kb range) that is suitable for restriction endonuclease digestion; plasmid, phage, or cosmid subcloning; end-fragment isolation; PCR; and random labeling.
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References
Olson, M. V., Loughney, K., and Hall, B. D. (1979) Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci. J. Mol. Biol. 132, 387–410.
Philippsen, P., Stotz, A., and Scherf, C. (1991) DNA of Saccharomyces cerevisiae. Methods Enzymol. 194, 169–182.
Rose, M. D., Winston, F., and Hieter, P. (1990) Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 198.
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© 1996 Humana Press Inc.
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Silverman, G.A. (1996). Purification of YAC-Containing Total Yeast DNA. In: Markie, D. (eds) YAC Protocols. Methods in Molecular Biology™, vol 54. Humana Press. https://doi.org/10.1385/0-89603-313-9:65
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DOI: https://doi.org/10.1385/0-89603-313-9:65
Publisher Name: Humana Press
Print ISBN: 978-0-89603-313-9
Online ISBN: 978-1-59259-541-9
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