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YAC Protocols pp 1–11Cite as

Generation of Large Insert YAC Libraries

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 54))

Abstract

The introduction of yeast artificial chromosomes (YACs) as cloning vectors in 1987 has significantly advanced the analysis of complex genomes (1). The capability of cloning large DNA (100–2000 kb) as YACs has accelerated the construction of physical maps and contig building (a contiguous set of overlapping clones). YAC contigs now cover entire human chromosomes (i.e., Y and 21) (2,3) and small genomes (i.e., Schizosaccharomyces pombe) (4), and large YAC contigs cover much of the human genome (5). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity and ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries (6). Therefore, YACs complement existing cloning vectors (cosmids, bacteriophage) and new cloning vectors (P1 bacteriophage [P1], bacterial artificial chromosomes [BACs], and P1-derived artificial chromosomes [PACs]; for review, see ref. 7) in mapping and chromosome walking projects (6, 8).

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Oxford University, Oxford, UK

    Zoia Larin

  2. Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK

    Anthony P. Monaco

  3. Max Planck Institute for Molecular Genetics, Berlin, Germany

    Hans Lehrach

Authors
  1. Zoia Larin
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  2. Anthony P. Monaco
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  3. Hans Lehrach
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Editor information

Editors and Affiliations

  1. Paediatric Research Unit, United Medical and Dental Schools of Guy’s and St. Thomas’ Hospitals, London, UK

    David Markie

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© 1996 Humana Press Inc.

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Larin, Z., Monaco, A.P., Lehrach, H. (1996). Generation of Large Insert YAC Libraries. In: Markie, D. (eds) YAC Protocols. Methods in Molecular Biology™, vol 54. Humana Press. https://doi.org/10.1385/0-89603-313-9:1

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  • DOI: https://doi.org/10.1385/0-89603-313-9:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-313-9

  • Online ISBN: 978-1-59259-541-9

  • eBook Packages: Springer Protocols

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