Purification of YAC-Containing Total Yeast DNA

  • Gary A. Silverman
Part of the Methods in Molecular Biology™ book series (MIMB, volume 54)

Abstract

There are many methods describing the preparation of DNA from Saccharomyces cerevisiae. The method described in this chapter is a modification of those described by Olson et al. (1) and Philippsen et al. (2). Although simpler techniques are available (3), this method routinely provides relatively high molecular weight DNA (50–250 kb range) that is suitable for restriction endonuclease digestion; plasmid, phage, or cosmid subcloning; end-fragment isolation; PCR; and random labeling.

Keywords

Glycerol Phenol EDTA Citrate Chloroform 

References

  1. 1.
    Olson, M. V., Loughney, K., and Hall, B. D. (1979) Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci. J. Mol. Biol. 132, 387–410.PubMedCrossRefGoogle Scholar
  2. 2.
    Philippsen, P., Stotz, A., and Scherf, C. (1991) DNA of Saccharomyces cerevisiae. Methods Enzymol. 194, 169–182.PubMedCrossRefGoogle Scholar
  3. 3.
    Rose, M. D., Winston, F., and Hieter, P. (1990) Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 198.Google Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Gary A. Silverman
    • 1
  1. 1.Department of PediatricsHarvard Medical SchoolBoston

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