Purification of YAC-Containing Total Yeast DNA
There are many methods describing the preparation of DNA from Saccharomyces cerevisiae. The method described in this chapter is a modification of those described by Olson et al. (1) and Philippsen et al. (2). Although simpler techniques are available (3), this method routinely provides relatively high molecular weight DNA (50–250 kb range) that is suitable for restriction endonuclease digestion; plasmid, phage, or cosmid subcloning; end-fragment isolation; PCR; and random labeling.
KeywordsGlycerol Phenol EDTA Citrate Chloroform
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