YAC Protocols pp 281-292 | Cite as

YAC Transfer into Mammalian Cells by Cell Fusion

  • Nicholas P. Davies
  • Clare Huxley
Part of the Methods in Molecular Biology™ book series (MIMB, volume 54)


The large regions of DNA that can be cloned in yeast artificial chromosomes (YACs) are ideal for expression studies of the complex genes and gene clusters found in the mammalian genome. Such studies require that the YAC of interest be transferred into a suitable expression system, such as mammalian cells in tissue culture or transgenic animals. Recent experiments indicate that large genes cloned on YACs may be transferred intact and are often expressed at a level comparable to the endogenous genes and in a fully controlled fashion owing to the large amount of flanking DNA containing long range controlling elements (reviewed in ref. 1).


Mammalian Cell Selectable Marker Yeast Artificial Chromosome Extrachromosomal Element Rodent Cell Line 
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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Nicholas P. Davies
    • 1
  • Clare Huxley
    • 1
  1. 1.Department of Biochemistry and Molecular GeneticsSt. Mary’s Hospital Medical SchoolLondonUK

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