YAC Protocols pp 145-155 | Cite as

End-Rescue of YAC Clone Inserts by Inverse PCR

  • Gary A. Silverman
Part of the Methods in Molecular Biology™ book series (MIMB, volume 54)


The termini of yeast artificial chromosome (YAC) inserts can serve as probes or sequence tagged sites (STSs) that are useful in genomic analysis. For example, these elements can be used to:
  1. 1.

    Build physical maps and YAC contigs;

  2. 2.

    Characterize and orient YAC clones;

  3. 3.

    Assess chromosomal origin and YAC chimerism; and

  4. 4.

    Construct conventional and rare-cutting restriction maps.



Polymerase Chain Reaction Polymerase Chain Reaction Product Yeast Artificial Chromosome Inverse Polymerase Chain Reaction Yeast Artificial Chromosome Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Silverman, G. A. (1993) Isolating vector-insert junctions from yeast artificial chromosomes. PCR Methods Appl. 3, 141–150.PubMedGoogle Scholar
  2. 2.
    Silver, J. and Keerikatte, V. (1989) Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus. J. Virol. 63, 1924–1928. (Published erratum appears in J. Virol. 1990, 64[6], 3150.)PubMedGoogle Scholar
  3. 3.
    Triglia, T., Peterson, M. G., and Kemp, D. J. (1988) A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16, 8186.PubMedCrossRefGoogle Scholar
  4. 4.
    Ochman, H., Gerber, A. S., and Hartl, D. L. (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120, 621–623.PubMedGoogle Scholar
  5. 5.
    Silverman, G. A., Ye, R. D., Pollock, K. M., Sadler, J. E., and Korsmeyer, S. J. (1989) Use of yeast artificial chromosome clones for mapping and walking within human chromosome segment 18q21.3. Proc. Natl. Acad. Sci. USA 86, 7485–7489.PubMedCrossRefGoogle Scholar
  6. 6.
    Silverman, G. A., Jockel, J. I., Domer, P. H., Mohr, R. M., Taillon, M. P., and Korsmeyer, S. J. (1991) Yeast artificial chromosome cloning of a two-megabase-size contig within chromosomal band 18q21 establishes physical linkage between BCL2 and plasminogen activator inhibitor type-2. Genomics 9, 219–228.PubMedCrossRefGoogle Scholar
  7. 7.
    Zuo, J., Robbins, C., Taillon, M. P., Cox, D. R., and Myers, R. M. (1992) Cloning of the Huntington disease region in yeast artificial chromosomes. Hum. Mol. Genet. 1, 149–159.PubMedCrossRefGoogle Scholar
  8. 8.
    Groden, J., Thliveris, A., Samowitz, W., Carlson, M., Gelbert, L., Albertsen, H., et al. (1991) Identification and characterization of the familial adenomatous polyposis coli gene. Cell 66, 589–600.PubMedCrossRefGoogle Scholar
  9. 9.
    Joslyn, G., Carlson, M., Thliveris, A., Albertsen, H., Gelbert, L., Samowitz, W., et al. (1991) Identification of deletion mutations and three new genes at the familial polyposis locus. Cell 66, 601–613.PubMedCrossRefGoogle Scholar
  10. 10.
    Grill, E. and Somerville, C. (1991) Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking. Mol. Gen. Genet. 226, 484–490.PubMedCrossRefGoogle Scholar
  11. 11.
    Rose, M. D., Winston, F., and Hieter, P. (1990) Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, p. 198.Google Scholar

Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Gary A. Silverman

There are no affiliations available

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