YAC Protocols pp 145-155 | Cite as

End-Rescue of YAC Clone Inserts by Inverse PCR

  • Gary A. Silverman
Part of the Methods in Molecular Biology™ book series (MIMB, volume 54)

Abstract

The termini of yeast artificial chromosome (YAC) inserts can serve as probes or sequence tagged sites (STSs) that are useful in genomic analysis. For example, these elements can be used to:
  1. 1.

    Build physical maps and YAC contigs;

     
  2. 2.

    Characterize and orient YAC clones;

     
  3. 3.

    Assess chromosomal origin and YAC chimerism; and

     
  4. 4.

    Construct conventional and rare-cutting restriction maps.

     

Keywords

EDTA Agarose Bromide Electrophoresis MgCl2 

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Copyright information

© Humana Press Inc. 1996

Authors and Affiliations

  • Gary A. Silverman

There are no affiliations available

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