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The Loose Patch Voltage Clamp Technique

  • Protocol
Patch-Clamp Applications and Protocols

Part of the book series: Neuromethods ((NM,volume 26))

Abstract

Extracellular microelectrodes have been used for many years to apply focal electrical stimulation to individual cells (for example, see Pratt and Eisenberger [1919] and Huxley and Taylor [1958]). Strickholm (1961) was the first to use a single extracellular electrode for both voltage control and recording membrane current. Strickholm used this method (developed as part of his PhD thesis) to study muscle membrane impedance and capacitance. Strickholm’s method lay dormant for over 20 yr until it was revived by Stühmer and Almers (1982). Almers, Stühmer, and their collaborators used the loose patch-clamp to study a wide range of muscle sodium and potassium channel properties, including mobility, inactivation, and spatial distribution (Stühmer and Almers, 1982; Almers et al., 1983a,b, 1984; Stühmer et al., 1983; Roberts and Almers, 1984,1992; Weiss et al., 1986; Roberts, 1987).

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Author information

Authors and Affiliations

  1. Departments of Cellular and Structural BioZogy and Physiology, and the Neuroscience Program, University of Colorado School of Medicine, Denver, CO

    J. H. Caldwelland

  2. Indiana University School of Medicine, Muncie Centerfor Medical Education, Bull State University, Muncie, IN

    R. L. Milton

Authors
  1. J. H. Caldwelland
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  2. R. L. Milton
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Editor information

Editors and Affiliations

  1. Uniuersity of Saskatchewan, Saskatoon, Canada

    Alan A. Boulton  & Wolfgang Walz  & 

  2. Unioersity of Alberta, Edmonton, Canada

    Glen B. Baker

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© 1995 The Humana Press Inc.

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Caldwelland, J.H., Milton, R.L. (1995). The Loose Patch Voltage Clamp Technique. In: Boulton, A.A., Baker, G.B., Walz, W. (eds) Patch-Clamp Applications and Protocols. Neuromethods, vol 26. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-311-2:173

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  • DOI: https://doi.org/10.1385/0-89603-311-2:173

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-311-5

  • Online ISBN: 978-1-59259-631-7

  • eBook Packages: Springer Protocols

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