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Electroporation of Dictyostelium discoideum

  • Protocol
Electroporation Protocols for Microorganisms

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 47))

Abstract

Dictyostelium discoideum has long been an intriguing model system for the study of cell type divergence during development, cell signaling, gene expression, and other cell biological problems (1). With the development of DNA-mediated transformation, research in Dictyostelium has entered a new era. The ability to introduce molecules into cells has allowed the analysis of promoters by transient and stable expression of reporter genes, such as luciferase and β-galactosidase (2,3). Stable transformants have also been created that overexpress normal and altered copies of important structural and regulatory proteins (4–9). Most importantly, the development of homologous gene targeting and antisense RNA inhibition of gene expression has allowed the creation of mutant cell lines lacking specific gene products (10,11). These techniques, coupled with the recently created ordered yeast artificial chromosome library, will allow large-scale mapping, fine structure mapping, and eventually sequencing of the entire genome (12).

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT

    David Knecht & Ka Ming Pang

Authors
  1. David Knecht
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  2. Ka Ming Pang
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Editors and Affiliations

  1. Department of Cancer Biology, Harvard University School of Public Health, Boston, MA

    Jac A. Nickoloff

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© 1995 Humana Press Inc.

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Knecht, D., Pang, K.M. (1995). Electroporation of Dictyostelium discoideum . In: Nickoloff, J.A. (eds) Electroporation Protocols for Microorganisms. Methods in Molecular Biology™, vol 47. Humana Press. https://doi.org/10.1385/0-89603-310-4:321

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  • DOI: https://doi.org/10.1385/0-89603-310-4:321

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-310-8

  • Online ISBN: 978-1-59259-534-1

  • eBook Packages: Springer Protocols

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